The main purpose of the current study was to assess the effect of gender on the proficiency of teaching. To achieve this objective, the researcher used descriptive survey design. All the teacher trainees of colleges of education in Jammu province constituted the population for this the random sampling technique was used to select the sample of the study. 120 teacher trainees from 5 districts of Jammu province were taken for the final sample. The researched employed Mean, Standard Deviation and T-test to analyze the data. From the findings of the data analysis it is concluded that effect of gender on the proficiency in teaching stands neutral. Furthermore in various dimensions of proficiency in teaching there is also insignificance difference in proficiency in teaching as far as the effect of gender is concerned. The study also reveals that the proficiency in teaching is high in both the genders among the teacher trainees of Jammu province.