GSTs are critical in the detoxification of drugs, carcinogens, and reactive oxygen species, The genetic polymorphisms of GSTs enzymes have an etiological role in breast cancer. The aim of this study was to evaluate relation of glutathione S-transferases in the pathophysiology of breast cancer disease. The genetic investigation was done PCR, Gel electrophoresis and RFLP. The frequencies of GSTA1 genotypes in breast cancer patients were 76 % AA (mutant type) and 24% GG (wild type) while in healthy subjects 55% GG (wild type) and 45% AA (mutant type). Mutant type genetic polymorphism is related to Breast Cancer Patients.