This study aimed to evaluate the effectiveness of ultrasound in detecting changes in teat and supra mammary lymph node parameters in dairy cows with mastitis. The study focused on teat width, teat wall thickness, teat canal length, and supra mammary lymph node size, using ultrasound to scan 48 udder quarters and 28 lymph nodes of sub-clinical, and clinical groups respectively. The findings revealed that ultrasonography can identify clinical mastitis and provide valuable additional information on the udder's condition. Mastitis caused a considerable thickening of the teat wall and a loss of anechoic zones in the cistern. In contrast, the lymph nodes on the mammary gland's bacteriologically positive side were significantly larger and more hypoechogenic than those on the opposing side. The study concludes that ultrasound can be a useful tool in diagnosing mastitis in dairy cows and in evaluating the health of the mammary gland.