Pyrolysis of Eriobotrya japonica seed oil was investigated in steel-made fixed bed reactor with (Alumina, Red mud, and Bentonite clay) and without catalyst at three different temperatures. The influence of pyrolysis parameters such as catalyst and temperature on the percentage yield was studied. It was found that temperature, as well as catalyst, affect the percentage yield of pyrolytic products. Results indicate that yield of bio-oil was significantly improved while using red mud and bentonite clay but remarkable results were obtained with alumina due to the presence of acidic sites. However, it was further observed that at 550°C the aromatic content increased considerably with alumina. Additionally, bio-oil obtained at different temperatures (350°C and 550°C) was investigated by elemental analysis and GC-MS, and about 44 and 53 different compounds were identified in liquid products.