This study intends to examine the association between gender and causative factors affecting gender discrimination in Punjab. Several women throughout the world live in conditions in which they are deprived of their basic human rights for no other reason than their sex. A cross-sectional survey has been conducted and a sample of 200 men and women has been selected using snowball sampling technique from the district of Gujrat, Punjab, Pakistan. A set of variables have been used as the determinants of gender discrimination and a structured questionnaire has been administered. Further, it was pretested from 30 selected participants and the value of Alpha ranged from .709 to .885. Moreover, statistical analysis has been made to conclude the findings. The study findings outlined that there is an association between gender with favor, reason, employment opportunity, health issues, workplace, rights, family setups, and role in the elimination of gender discrimination. Similarly, the study also depicted that gender has significant differences in response to favor, reason, religion, working place, rights, employment opportunity, and health issues.