Background: During the last few decades, because of the use of antiretroviral medication, Human Immunodeficiency Virus (HIV) has converted to a chronic illness rather than a disease that quickly directs to severe sickness and death. Due to antiretroviral therapy (ART), HIV-infected patients' life expectancy has been increased; spouse training can play a vital role. 

Objective: To assess the quality of life (QOL) and antiretroviral therapy (ART) adherence among PLWHIV after spouses training in nursing skills. 

Methods: A randomized control trial was conducted. Blood test for HIV was done for 250 families, out of which 60 females were selected whose spouse's test was positive for HIV on Elisa's kit while they were HIV negative. The experimental group was trained in nursing skills, which consists of care for wounds and their prevention, hand washing and nail care, care of mouth, care of body wash, hair wash and towels, comb, and toothbrushes. 

Result: There were 30 patients in the control group and 23 in the intervention group. The proportion of education type in the intervention group was not entirely different; however, in the control group, most of the patients were illiterate or primarily educated. There were 86.7% symptomatic in the control group and 43.5% and 56.5% asymptomatic, respectively, and AID converted in the intervention group. 

Conclusion: The nursing training for spouses of HIV patients improves the quality of life of HIV-affected patients and also adheres to ART. Regular assessments of health-related quality of life may deliver significant indicators for monitoring and assessing HIV/AIDS services.