This research aimed to find antibacterial, thrombolytic, cytotoxic and antioxidant potential of different extracts (ethanol, methanol, ethyl acetate) of Withania coagulans seeds. Anti-bacterial activity was carried out through well diffusion method. Thrombolytic activity was determined by the percentage of clot lysis. Cytotoxic activity was performed through hemolytic method. ntioxidant activity of extracts was determined by using 2, 2-diphenyl- 1-picrylhydrazyl radical scavenging assay, peroxidase (POD) assay, superoxide dismutase (SOD) assay and catalase (CAT) assay. Total phenolic and flavonoid contents were determined. Ethanol extract exhibited maximum antibacterial effects (p>0.001) against both strains as compared to methanol and ethyl acetate extracts. All the extract showed concentration dependent thrombolytic activity. Ethanolic extract of showed maximum %age of clot lysis followed by methanol and then ethyl acetate extract. Cytotoxic activity when performed indicated that among all extracts Methanol extract of seeds showed maximum cytotoxic activity. The ethanol extract of seeds showed maximum total phenolic contents and total flavonoids contents. When DPPH free radical scavenging assay was carried ethanol extract of seeds of W. coagulans plant showed maximum %age inhibition followed by methanol extract and then Ethyl acetate extract. Similarly, ethanol extract showed maximum Peroxidase assay, superoxide dismutase, Catalase activities. Thus, results of study showed that different extracts ethanol, methanol and ethyl acetate of Withania coagulans seeds exhibited antimicrobial, cytotoxic and antioxidant potential, however, among the extracts ethanol possess the maximum therapeutic potential against all activities.