Objective: To study the effects of high-intensity interval training on lipid metabolism and serum inflammatory factors in overweight girls. Methods: An awareness campaign was conducted in an elementary school to recruit 10 overweight female students as experimental participants. An 8-week exercise program using the RUNNER (RUN-7410) for running table exercise was implemented with 3 to 4 workouts per week. Upon completion of the training, participants' body morphology data and blood samples will be collected for use in the study.Results: 1. The body shape of 10 overweight girls changed significantly after the intensity interval exercise intervention. Body weight decreased from 42.35±3.79kg to 39.93±3.21kg, body mass index (BMI) decreased from 22.76±1.34kg/m2 to 21.87±1.05kg/m2, and body fat percentage decreased from 34.05±2.86% to 30.06±2.05% with statistically significant differences (P<0.01). Waist circumference decreased from 51.25±3.51cm to 48.96±3.34cm, hip circumference decreased from 73.61±2.51cm to 71.58±2.52cm before the intervention, and waist-hip ratio decreased from 0.85±0.23 to 0.84±0.21 before the intervention; 2. Before the training and intervention, the overweight girls' TC, LDL-C, and HDL-C were within the normal range. However, TG had exceeded the normal level, indicating a mild lipid metabolism disorder. The lipid metabolism of obese overweight girls changed after the high-intensity interval training intervention, with TC decreasing from 3.24±0.28 mmol/L to 3.11±0.39, TG decreasing from 1.83±0.32 to 1.69±0.37 mmol/L, LDL-C decreasing from 2.06±0.31 to 1.81±0.42 mmol/L, HDL-C decreasing from 2.06±0.31 to 1.81±0.42 mmol/L, and HDL-C decreasing from 2.06±0.31 to 1.81±0.42 mmol/L. The results indicated that there were mild lipid metabolism disorders in obese girls. mmol/L, and HDL-C changed significantly (P<0.05) from 1.03±0.18 mmol/L to 1.25±0.27 mmol/L. 3. The levels of IL-6 and hs-CRP in the overweight girls before the exercise intervention had exceeded the normal range, indicating that an inflammatory response already existed in the organism. The IL-6 level of overweight girls before and after eight weeks of high-intensity interval training decreased from 34.47±2.52pg/ml to 29.63±3.15pg/ml, and the hs-CRP level decreased from 11.01±0.85mg/L to 9.03±1.02mg/L. Very significant changes were achieved in IL-6, hs-CRP before and after exercise intervention (P<0.01) . And after the intervention, the levels of IL-6, hs-CRP were normalized, and the inflammatory response of the organism disappeared.Conclusions: 1. The eight-week training program showed excellent results in reducing total body weight and body fat percentage, accompanied by a significant reduction in waist and hip circumference, which strongly proved the positive effects of high-intensity interval training in reshaping body shape and promoting weight management; 2. Eight weeks of high-intensity interval training reduced TG, TC, and LDL-C levels, and increased HDL-C levels in overweight girls, which revealed the This reveals the important value of high-intensity interval training in optimizing the balance of lipid metabolism; 3. Overweight girls showed mild inflammatory reactions, and serum IL-6 and hs-CRP levels have exceeded normal values. After 8 weeks of rigorous and regular high-intensity interval training, the levels of these inflammatory markers were significantly reduced to lower levels, which strongly proved that long-term adherence to this type of training can effectively inhibit the chronic inflammatory response triggered by obesity.