This study aims to assess the effectiveness of using UNO cards as a teaching aid in accounting education by comparing pre-test and post-test scores. The research design employed a before-and-after experimental approach. The study population comprised 244 students enrolled in the accounting vocational program across three grade levels, with a reachable population of 172 students in class X. Proportional simple random sampling was utilized to select a sample of 60 students, who subsequently completed pre-tests and post-tests using a motivation questionnaire. Data were quantitatively analyzed using a Likert scale and gain score analysis. Descriptive statistical results revealed a significant increase in students' learning motivation, with a post-test mean score of 91.13 compared to the pre-test mean score of 70.60. The majority of students exhibited a moderate level of motivation (58.3%), followed by high motivation (41.7%). Additionally, the data distribution was found to be normal for both pre-test and post-test scores. The paired sample t-test indicated a significant difference between the pre-test and post-test scores (p<0.001), suggesting a positive impact of using UNO cards as a learning medium. These findings suggest that the innovative and engaging nature of UNO cards contributes to heightened student motivation and active participation in accounting education. In conclusion, the incorporation of UNO cards as a teaching aid in accounting education effectively increased students' learning motivation. The use of innovative and engaging learning media, such as UNO cards, is recommended to enhance students' interest and enthusiasm in the learning process.