This research conducted in Can Tho City aims to discern the factors influencing consumers' decisions to use biofuel, with the ultimate goal of proposing impactful policies to encourage biofuel consumption. Employing quantitative methods, including descriptive statistics, Cronbach's Alpha test, exploratory factor analysis, and binary logistic regression, the study delves into consumers' environmental concerns and preferences. Findings highlight air pollution as the foremost environmental issue, with water and soil pollution following. The regression model identifies significant positive correlations between variables such as Environmental concern, Usefulness, Product knowledge, Seller, and educational level with consumers' biofuel usage decisions. Among these, Seller exerts the most substantial influence. The study suggests key solutions, including robust information dissemination policies, comprehensive training for gas station staff, encouragement of biofuel use among producers, government interventions in the automotive industry, quality management policies, capacity building through international collaboration, and an emphasis on ensuring both quantity and quality at fuel retailers. These proposals aim to address consumer concerns, enhance awareness, and establish trust in biofuel, laying the groundwork for its widespread adoption in Can Tho City.