In Vivo Ameliorative Impact Of Demethoxycurcumin, Epi-Orientin And Ursolic Acid On Magnesium Chloride Induced Multiple Organ Damage In Albino Wistar Rats

Abstract

The main and fastest reaction that infections use as a quick defense response is inflammation. A strong link has been established between inflammation and cancer. Animals and humans may have specific endocrine, hematological, cardiovascular, and neurological consequences from elevated serum magnesium concentrations. Magnesium compounds in mammalian cells produced aneuploidy, sister chromatid exchange, and DNA strand breaks, but they did not result in chromosomal abnormalities. Moreover demethoxycurcumin, EPI-orientin and ursolic acid produce antioxidant and hypoglycemic properties. In current study, the anti-inflammatory behavior of demethoxycurcumin, EPI-orientin, and ursolic acid has been examined after the induction of multiple organ toxicities via 100 mg/mL of MgCl₂, by keeping Naproxen @125mg/5mL/Kg/b.wt as standard in albino Wistar rats (150-200 g) of both genders while blood biochemistry has been done by ELISA approach. Results showed that female rats treated with demethoxycurcumin,  EPI-orientin, and ursolic acid showed a considerable improvement (p>0.005) in the levels of AST, ALT, bilirubin, albumin, globulin, ALP, BUN, creatinine, uric acid, haemoglobin, WBC, RBC, HCT, MCV, MCH, MCHC, platelets, neutrophils, eosinophils, lymphocytes and monocytes, especially at the concentration of 250 mg/mL/ Kg. Female rats showed significant changes in ALP, BUN, and AST (P ˂ 0.01), but no significant changes in ALT were seen when compared to the control group. At 100 mg/ Kg, these compounds produced significant change in cholesterol, HDL, LDL, triglycerides, and VLDL concentrations (P ˂ 0.05) as compared to compared to the rats of control group at all concentrations of compound. Moreover, histopathological findings (at 100 µm) showed that MgCl₂ induced severe toxicity in the liver, kidney, and heart. Therefore, the combinational formulation of the three bioactive components (demethoxycurcumin, EPI-orientin and ursolic acid) was found to be the best regimen for obtaining a 95% recovery from multiple organ damage so these compounds can be used, alone and in combination, to limit multiple organ damage due to over dose of megensium (which is used in different pharmaceutical products