Effect Of Rki-1447 On The Behavioral Aspect Post Compression Injury Model In Rats

Abstract

Objective: In this study a standardized spinal cord compression injury model with application of RKI-1447 to see whether RKI-1447 has the capacity to alleviate SCI related sensori-motor/ behavioral responses.

Methods: Sixty-four adult Sprague Dawley rats were used. The rats were divided into four groups A (Control group with only skin incision), B, C and D groups (all underwent laminectomy followed by compression injuries by applying the aneurysm clip of 70 gm for 01 minute. In addition, group C and D were given different doses of RKI-1447 (as 0.3 µg and 0.6 µg respectively). The animals were observed for sensorimotor behavioral responses on day 7, 14 and 28 post surgically using Basso, Beattie, Bresnahan (BBB) test, Cold sensation test, Von frey filaments, Hot plate test and tail flick test on each rats.

Results: The results acquired from all the groups were statistically analyzed by using repeated measure ANOVA followed by Tukey HSD test for groups comparison. Overall, the group A showed no neurological abnormalities, whereas the group B exhibited a clear neurological deficit, and group C and D showed significant improvements in their behavioral activities, comparable to group  B at all evaluated time points (p≤0.05).

Conclusion: RKI-1447 appeared to improve the sensory as well as motor behavior of the rat undergoing spinal cord compression injury. Our results suggest a promising role for RKI-1447 in alleviating SCI-related impairments, offering potential avenues for further exploration and therapeutic development.