Prevalence And Molecular Profiling Of Antibiotics Resistance Genes In Bacterial Isolates

Authors

  • Dr. Sudhair Abbas Bangash
  • Madeeha Arshad
  • Rabia Yaqoob
  • Dr Ravi Dutt Sharma
  • Dr Irum Javid
  • Dr Tamsal Murtza
  • Syed Shahab Ud Din Shah
  • Inam-u-llah

DOI:

https://doi.org/10.53555/ks.v12i5.3441

Keywords:

Antibiotic resistance, multidrug-resistant bacteria, molecular profiling, blaCTX-M, blaNDM-1, mecA, antimicrobial resistance

Abstract

Background: One of the biggest threats to world health is antibiotic resistance, especially in areas with little resources. In this work, bacterial isolates from Hayatabad Medical Complex (HMC), Peshawar, were molecularly profiled for resistance genes in order to assess the incidence of antibiotic resistance.

Objectives: The research aimed to ascertain the prevalence of multidrug-resistant (MDR) bacterial isolates and to detect the presence of important antibiotic resistance genes in these isolates.

Methods: A one-year cross-sectional investigation containing 210 bacterial isolates from different clinical samples (blood, urine, sputum, and wound swabs) was carried out, concluding in March 2024. To test for antibiotic susceptibility, the Kirby-Bauer disk diffusion technique was used. PCR was used to do molecular profiling of resistance genes, such as blaCTX-M, blaNDM-1, mecA, and others. To assess the relationship between resistance genes and phenotypic resistance patterns, statistical analysis was done.

Results: According to the research, 42% of the bacterial isolates were MDR and 65% of the isolates showed resistance to at least one class of antibiotics. The most prevalent kind of resistance was to β-lactams, especially in Klebsiella pneumoniae and Escherichia coli. Using molecular profiling, it was possible to identify blaNDM-1 in 10% of Klebsiella pneumoniae and 7% of Pseudomonas aeruginosa, and blaCTX-M in 48% of E. coli and 40% of Klebsiella pneumoniae. Antibiotic resistance patterns and the existence of resistance genes were shown to be significantly correlated (p < 0.01).

Conclusion: The high frequency of multidrug resistance and the significance of molecular diagnostics in the treatment of antibiotic resistance are highlighted by this research. Improved antibiotic stewardship and ongoing molecular monitoring are desperately needed.

Author Biographies

Dr. Sudhair Abbas Bangash

Faculty of Life Science, Department of Pharmacy, Sarhad University of Science and Information Technology, Peshawar, Pakistan. 

Madeeha Arshad

Department of Zoology, Division of Science and Technology, University of Education Lahore. Email: 

Rabia Yaqoob

Department of Zoology, Division of Science and Technology, University of Education Lahore. Email: 

Dr Ravi Dutt Sharma

Assistant Professor-Biology, Department of Pure Sciences, College of Engineering, Science and Technology. Natabua Campus, Lautoka. Fiji National University, Republic of Fiji.

Dr Irum Javid

Assistant Professor, Department of Biochemistry, Sardar Bahadur Khan Women University, Quetta Pakistan

Dr Tamsal Murtza

Department of Plant Pathology, University of Agriculture, Faisalabad.

 

Syed Shahab Ud Din Shah

Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan

 

Inam-u-llah

Department of Food Science and Technology, The University of Haripur. Pakistan

 

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Published

2024-09-26

How to Cite

Dr. Sudhair Abbas Bangash, Madeeha Arshad, Rabia Yaqoob, Dr Ravi Dutt Sharma, Dr Irum Javid, Dr Tamsal Murtza, … Inam-u-llah. (2024). Prevalence And Molecular Profiling Of Antibiotics Resistance Genes In Bacterial Isolates. Kurdish Studies, 12(5), 1152–1157. https://doi.org/10.53555/ks.v12i5.3441